Article
Method for fluorescent marker swapping and its application in Steinernema nematode colonization studies.
Journal of microbiological methods - 1 Jun 2015
Gengler Samuel, Batoko Henri, Wattiau Pierre
Abstract excerpt
An allelic exchange vector was constructed to replace gfp by mCherry in bacteria previously tagged with mini-Tn5 derivatives. The method was successfully applied to a gfp-labeled Yersinia pseudotuberculosis strain and the re-engineered bacterium was used to study the colonization of Steinernema nematodes hosting their Xenorhabdus symbiont using dual-color confocal microscopy.
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