Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Construction of a Sinorhizobium meliloti strain carrying a stable and non-transmissible chromosomal single copy of the green fluorescent protein GFP-P64L/S65T.

FEMS microbiology letters - 10 Sept 2002

Pistorio M, Balagué L J, Del Papa M F, Pich-Otero A, Lodeiro A, Hozbor D F, Lagares A

Abstract excerpt

A single copy of the green fluorescent protein (GFP)-encoding gene gfp-P64L/S65T under the control of the constitutive nptII promoter was introduced in a neutral region of the Sinorhizobium meliloti chromosome, between the genes recA and alaS. Within the same chromosomal region downstream of gfp-P64L/S65T a tetracycline (Tc) resistant cassette was also inserted. Both markers were very stable during at least 40...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Chromosomes, Bacterial
Share to Fluorescence

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genetic Vectors

Share to Luminescent Proteins

Share to Nitrogen Fixation

Share to Sinorhizobium meliloti

Share to Soil Microbiology

Share to Symbiosis

Source

Identifiers

PMID: 12351225
DOI: 10.1111/j.1574-6968.2002.tb11341.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Construction of a Sinorhizobium meliloti strain carrying a stable and non-transmissible chromosomal single copy of the green fluorescent protein GFP-P64L/S65T.PMID 12351225Read ↗

Versatile Vectors for Efficient Mutagenesis of Bradyrhizobium diazoefficiens and Other Alphaproteobacteria.Semantic proximity Read ↗

Comparative genomic analysis of Sinorhizobium meliloti LPU88: plasmid diversity and conjugative mechanisms.Semantic proximity Read ↗

The Plasmid Mobilome of the Model Plant-Symbiont Sinorhizobium meliloti: Coming up with New Questions and Answers.Semantic proximity Read ↗

Construction of green fluorescent protein (GFP)-marked strains of Bradyrhizobium for ecological studies.Semantic proximity Read ↗

The Sinorhizobium meliloti essential porin RopA1 is a target for numerous bacteriophages.Semantic proximity Read ↗

Characterization of two bioluminescent Rhizobium meliloti strains constructed for field releases.Semantic proximity Read ↗

A chromosomal insertion toolbox for promoter probing, mutant complementation, and pathogenicity studies in Ralstonia solanacearum.Semantic proximity Read ↗

Identification of genes relevant to symbiosis and competitiveness in Sinorhizobium meliloti using signature-tagged mutants.Semantic proximity Read ↗

Stationary-phase mutants of Sinorhizobium meliloti are impaired in stationary-phase survival or in recovery to logarithmic growth.Semantic proximity Read ↗

Exploring the symbiotic pangenome of the nitrogen-fixing bacterium Sinorhizobium meliloti.Semantic proximity Read ↗

Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid.Semantic proximity Read ↗

Spontaneous Mutations in the Nitrate Reductase Gene napC Drive the Emergence of Eco-friendly Low-N2O-Emitting Alfalfa Rhizobia in Regions with Different Climates.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.