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Transformation of Sclerotinia sclerotiorum with the green fluorescent protein gene and fluorescence of hyphae in four inoculated hosts

2009-06-01

Abstract excerpt

To obtain a genetic marker to observe and study the interaction of Sclerotinia sclerotiorum with its hosts, isolates ND30 and ND21 were transformed using pCT74 and gGFP constructs, both containing genes for the green fluorescent protein (GFP) and hygromycin B phosphotransferase. Putative transformants were obtained using polyethylene glycol-mediated transformation of protoplasts. Seven stable gfp transformants wer...

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Literature Corpus work
9c2ce280-1fbb-5d88-8acf-503e1979085a
DOI
10.1111/j.1365-3059.2009.02022.x
Open publication

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