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Article

The effects of sequence length and oligonucleotide mismatches on 5' exonuclease assay efficiency.

Nucleic acids research - 15 Oct 2002

Smith Steve, Vigilant Linda, Morin Phillip A

Abstract excerpt

Although increasingly used for DNA quantification, little is known of the dynamics of the 5' exonuclease assay, particularly in relation to amplicon length and mismatches at oligonucleotide binding sites. In this study we used seven assays targeting the c-myc proto-oncogene to examine the effects of sequence length, and report a marked reduction in efficiency with increasing fragment length. Three of the assays...

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Identifiers

PMID: 12384613
DOI: 10.1093/nar/gnf110
PMCID: PMC137155

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