Article
Distribution of 1000 sequenced T-DNA tags in the Arabidopsis genome.
The Plant journal : for cell and molecular biology - 1 Oct 2002
Szabados László, Kovács Izabella, Oberschall Attila, Abrahám Edit, Kerekes Irén, Zsigmond Laura, Nagy Réka, Alvarado Martha, Krasovskaja Inga, Gál Mónika, Berente Anikó, Rédei George P, Haim Amit Ben, Koncz Csaba
Abstract excerpt
Induction of knockout mutations by T-DNA insertion mutagenesis is widely used in studies of plant gene functions. To assess the efficiency of this genetic approach, we have sequenced PCR amplified junctions of 1000 T-DNA insertions and analysed their distribution in the Arabidopsis genome. Map positions of 973 tags could be determined unequivocally, indicating that the majority of T-DNA insertions landed in...
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