Article
PCR-based screening for insertional mutants.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2006
Stepanova Anna N, Alonso Jose M
Abstract excerpt
In Arabidopsis, where homologous recombination is highly inefficient, insertional mutagenesis has provided a great alternative means for identifying loss-of-function mutants in genes of interest. Many collections of T-DNA and transposon-tagged plants have been generated and several have been made available to the research community. With recent sequencing of the insertion sites and the creation of public...
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