Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Mapping multiple co-sequenced T-DNA integration sites within the Arabidopsis genome.

Bioinformatics (Oxford, England) - 22 Mar 2003

Presting Gernot G

Abstract excerpt

MOTIVATION: Insertion mutagenesis, using transgenes or endogenous transposons, is a popular method for generating null mutations (knockouts) in model organisms. Insertions are mapped to specific genes by amplifying (via TAIL-PCR) and sequencing genomic regions flanking the inserted DNA. The presence of multiple TAIL-PCR templates in one sequencing reaction results in chimeric sequence of intermittently low...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Arabidopsis
Share to DNA, Bacterial
Share to DNA Mutational Analysis

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA Transposable Elements

Share to Gene Expression Profiling

Share to Genome, Plant

Share to Sequence Alignment

Share to Sequence Homology

Source

Identifiers

PMID: 12651715
DOI: 10.1093/bioinformatics/btg049

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Mapping multiple co-sequenced T-DNA integration sites within the Arabidopsis genome.PMID 12651715Read ↗

Distribution of 1000 sequenced T-DNA tags in the Arabidopsis genome.Semantic proximity Read ↗

Time- and cost-efficient identification of T-DNA insertion sites through targeted genomic sequencing.Semantic proximity Read ↗

Genome-wide insertional mutagenesis of Arabidopsis thaliana.Semantic proximity Read ↗

A comprehensive characterization of single-copy T-DNA insertions in the Arabidopsis thaliana genome.Semantic proximity Read ↗

Restriction site extension PCR: a novel method for high-throughput characterization of tagged DNA fragments and genome walking.Semantic proximity Read ↗

Re-sequencing transgenic plants revealed rearrangements at T-DNA inserts, and integration of a short T-DNA fragment, but no increase of small mutations elsewhere.Semantic proximity Read ↗

A user's guide to the Arabidopsis T-DNA insertion mutant collections.Semantic proximity Read ↗

The Position and Complex Genomic Architecture of Plant T-DNA Insertions Revealed by 4SEE.Semantic proximity Read ↗

Rapid identification of Arabidopsis insertion mutants by non-radioactive detection of T-DNA tagged genes.Semantic proximity Read ↗

Identification of Arabidopsis thaliana transformants without selection reveals a high occurrence of silenced T-DNA integrations.Semantic proximity Read ↗

High-throughput TILLING for Arabidopsis.Semantic proximity Read ↗

Interactions among genomic structure, function, and evolution revealed by comprehensive analysis of the Arabidopsis thaliana genome.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.