Article
Genome-wide insertional mutagenesis of Arabidopsis thaliana.
Science (New York, N.Y.) - 1 Aug 2003
Alonso José M, Stepanova Anna N, Leisse Thomas J, Kim Christopher J, Chen Huaming, Shinn Paul, Stevenson Denise K, Zimmerman Justin, Barajas Pascual, Cheuk Rosa, Gadrinab Carmelita, Heller Collen, Jeske Albert, Koesema Eric, Meyers Cristina C, Parker Holly, Prednis Lance, Ansari Yasser, Choy Nathan, Deen Hashim, Geralt Michael, Hazari Nisha, Hom Emily, Karnes Meagan, Mulholland Celene, Ndubaku Ral, Schmidt Ian, Guzman Plinio, Aguilar-Henonin Laura, Schmid Markus, Weigel Detlef, Carter David E, Marchand Trudy, Risseeuw Eddy, Brogden Debra, Zeko Albana, Crosby William L, Berry Charles C, Ecker Joseph R
Abstract excerpt
Over 225,000 independent Agrobacterium transferred DNA (T-DNA) insertion events in the genome of the reference plant Arabidopsis thaliana have been created that represent near saturation of the gene space. The precise locations were determined for more than 88,000 T-DNA insertions, which resulted in the identification of mutations in more than 21,700 of the approximately 29,454 predicted Arabidopsis genes....
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