Article
Functional expression in vitro of bovine visual rhodopsin.
Protein engineering - 1 Apr 1990
Zozulya S A, Gurevich V V, Zvyaga T A, Shirokova E P, Dumler I L, Garnovskaya M N, Natochin MYu, Shmukler B E, Badalov P R
Abstract excerpt
Expression of functionally active bovine visual rhodopsin was achieved by sequential transcription and translation in vitro of rhodopsin gene cDNA with co-translational insertion of the protein into phosphatidylcholine liposomes. The recombinant rhodopsin has functional, spectral and immunochemical properties similar to those of natural rhodopsin from bovine retina. Two mutant rhodopsins, Cys316----Ser and...
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