Article
Functional expression in vitro of bovine visual rhodopsin.
Protein engineering - 1 Apr 1990
Zozulya S A, Gurevich V V, Zvyaga T A, Shirokova E P, Dumler I L, Garnovskaya M N, Natochin MYu, Shmukler B E, Badalov P R
Abstract excerpt
Expression of functionally active bovine visual rhodopsin was achieved by sequential transcription and translation in vitro of rhodopsin gene cDNA with co-translational insertion of the protein into phosphatidylcholine liposomes. The recombinant rhodopsin has functional, spectral and immunochemic...
Topics
- Animals
- Base Sequence
- Cattle
- Cloning, Molecular
- DNA
- Eye Proteins
- Gene Expression
- Genes
- Molecular Sequence Data
- Mutation
- Protein Biosynthesis
- Recombinant Proteins
- Restriction Mapping
- Retinal Pigments
- Rhodopsin
- Rod Opsins
- Transcription, Genetic
