Article
Oligosaccharide binding to barley alpha-amylase 1.
The Journal of biological chemistry - 23 Sept 2005
Robert Xavier, Haser Richard, Mori Haruhide, Svensson Birte, Aghajari Nushin
Abstract excerpt
Enzymatic subsite mapping earlier predicted 10 binding subsites in the active site substrate binding cleft of barley alpha-amylase isozymes. The three-dimensional structures of the oligosaccharide complexes with barley alpha-amylase isozyme 1 (AMY1) described here give for the first time a thorough insight into the substrate binding by describing residues defining 9 subsites, namely -7 through +2. These...
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