Article
Role of two dileucine-like motifs in insulin receptor anchoring to microvilli.
The Journal of biological chemistry - 15 Nov 2002
Shackleton Sue, Hamer Isabelle, Foti Michelangelo, Zumwald Nicole, Maeder Christine, Carpentier Jean-Louis
Abstract excerpt
In the absence of ligand, the insulin receptor is maintained on microvilli on the cell surface. A dileucine motif (LL(986-987)) is necessary but not sufficient for this anchoring, which also required the presence of additional sequence(s) downstream of position 1000. The aim of the present study was to identify this (these) additional sequence(s). First, exons 16 or 17 were fused to the extracellular and...
Topics
- Amino Acid Motifs
- Animals
- CHO Cells
- Cells, Cultured
- Cricetinae
- Cytoskeleton
- DNA, Complementary
- Exons
- Leucine
- Ligands
- Microscopy, Electron
- Microvilli
- Models, Biological
- Mutation
- Protein Binding
- Protein Conformation
- Protein Structure, Secondary
- Protein Structure, Tertiary