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Article

Direct in vivo screening of intrabody libraries constructed on a highly stable single-chain framework.

The Journal of biological chemistry - 22 Nov 2002

der Maur Adrian Auf, Zahnd Christian, Fischer Franziska, Spinelli Silvia, Honegger Annemarie, Cambillau Christian, Escher Dominik, Plückthun Andreas, Barberis Alcide

Abstract excerpt

Single-chain Fv antibody fragments (scFv) represent a convenient antibody format for intracellular expression in eukaryotic or prokaryotic cells. These so-called intrabodies have great potential in functional genomics as a tool to study the function of newly identified proteins in vivo, for example by binding-induced modulation of their activity or by blocking interactions with other proteins. However, the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 12215438
DOI: 10.1074/jbc.M205264200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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