Article
iFRET: an improved fluorescence system for DNA-melting analysis.
Genome research - 1 Sept 2002
Howell W Mathias, Jobs Magnus, Brookes Anthony J
Abstract excerpt
Fluorescence resonance energy transfer (FRET) is a powerful tool for detecting spatial relationships between macromolecules, one use of which is the tracking of DNA hybridization status. The process involves measuring changes in fluorescence as FRET donor and acceptor moieties are brought closer together or moved farther apart as a result of DNA hybridization/denaturation. In the present study, we introduce a new...
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