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Article

Guinea pig phospholipase B, identification of the catalytic serine and the proregion involved in its processing and enzymatic activity.

The Journal of biological chemistry - 15 Nov 2002

Nauze Michel, Gonin Lauriane, Chaminade Brigitte, Perès Christine, Hullin-Matsuda Francoise, Perret Bertrand, Chap Hugues, Gassama-Diagne Ama

Abstract excerpt

Guinea pig phospholipase B (GPPLB) is a glycosylated ectoenzyme of intestinal brush border membrane. It displays a broad substrate specificity and is activated by trypsin cleavage. The primary sequence contains four tandem repeat domains (I to IV) and several serines in lipase consensus sequences. We used site-directed mutagenesis to demonstrate that only the serine 399 present in repeat II is responsible for the...

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PMID: 12194976
DOI: 10.1074/jbc.M205761200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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