Article
Identification of the active site serine in pancreatic cholesterol esterase by chemical modification and site-specific mutagenesis.
The Journal of biological chemistry - 5 Oct 1990
DiPersio L P, Fontaine R N, Hui D Y
Abstract excerpt
Chemical modification and site-specific mutagenesis approaches were used in this study to identify the active site serine residue of pancreatic cholesterol esterase. In the first approach, purified porcine pancreatic cholesterol esterase was covalently modified by incubation with [3H]diisopropylf...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- Kinetics
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Pancreas
- Peptide Fragments
- Sterol Esterase
- Substrate Specificity
- Swine