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Article

T-DNA insertion in Arabidopsis caused coexisting chromosomal inversion and duplication at megabase level.

Gene - 25 Sept 2024

Gao Shuai, Huang Tian, Chen Lu, Jiang Ning, Ren Guodong

Abstract excerpt

Agrobacteria-mediated transformation is widely used in plant genetic engineering to introduce exogenous genes and create mutant lines through random T-DNA insertion and gene disruption. When T-DNA fragments are inserted into the plant genome, it could cause chromosomal abnormalities. In this study, we investigated the genetic basis of pleiotropic phenotypes observed in the T-DNA insertion mutant lnc161. We...

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Identifiers

PMID: 38762016
DOI: 10.1016/j.gene.2024.148577

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