Article
A transposon-based activation tagging system for gene function discovery in Arabidopsis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
Marsch-Martínez Nayelli
Abstract excerpt
Activation tagging is a powerful strategy to find new gene functions, especially from genes that are redundant or show lethal knock-out phenotypes. It has been applied using T-DNA or transposons. En/Spm-I/dSpm engineered transposons are efficient activation tags in Arabidopsis. An immobilized transposase source and an enhancer-bearing non-autonomous element are used in combination with positive and negative...
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