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Article

Increased proteolytic susceptibility of carboxypeptidase Y caused by modification of the disulfide zipper.

Bioscience, biotechnology, and biochemistry - 1 Jun 2002

Maki Toshihiko, Kozawa Hajime, Mima Joji, Ueno Hiroshi, Hayashi Rikimaru

Abstract excerpt

To investigate the structural importance of a "disulfide zipper" motif of carboxypeptidase Y, disulfide-deficient mutant enzymes were expressed in two strains of Saccharomyces cerevisiae. The mutant enzymes were rapidly degraded into fragments by intracellular proteases. Thus, it is concluded that the disulfide zipper is essential in maintaining the structural integrity of CPase Y against proteolytic susceptibility.

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Identifiers

PMID: 12162566
DOI: 10.1271/bbb.66.1393

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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