Article
Efficient homologous and illegitimate recombination in the opportunistic yeast pathogen Candida glabrata.
Genetics - 1 Mar 1999
Cormack B P, Falkow S
Abstract excerpt
The opportunistic pathogen Candida glabrata causes significant disease in humans. To develop genetic tools to investigate the pathogenicity of this organism, we have constructed ura3 and his3 auxotrophic strains by deleting the relevant coding regions in a C. glabrata clinical isolate. Linearized plasmids carrying a Saccharomyces cerevisiae URA3 gene efficiently transformed the ura3 auxotroph to prototrophy....
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