Article
A high-copy-number ADE2-bearing plasmid for transformation of Candida glabrata.
Gene - 12 May 1998
Hanic-Joyce P J, Joyce P B
Abstract excerpt
The Candida glabrata ADE2 gene encoding aminoimidazole ribonucleotide (AIR) carboxylase (EC 4.1.1.21) was isolated by complementation of the ade2-1 mutation in Saccharomyces cerevisiae. The predicted amino acid (aa) sequence is 75% identical to that of S. cerevisiae. Integrative transformation was used to produce a C. glabrata strain bearing a deletion of ADE2 coding sequences. A high-copy-number shuttle vector...
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