Article
Capillary electrophoretic detection in apolipoprotein E genotyping.
Electrophoresis - 1 Jun 2002
Bekers Otto, op den Buijsch Robert A M, de Vries Johan E, Wijnen Petal A H M, van Dieijen-Visser Marja P
Abstract excerpt
We describe the application of capillary electrophoresis to detect DNA fragments, obtained after amplifying a part of the apolipoprotein E (apoE) gene with polymerase chain reaction (PCR). Compared to conventional agarose slab gel electrophoresis (AGE), CE appears the method of choice with regard to resolution and sensitivity, to detect DNA fragments in the range of 20-100 base pairs. Especially discrimination...
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