Article
Capillary electrophoresis with laser-induced fluorescence detection for fast and reliable apolipoprotein E genotyping.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences - 25 Jul 2002
Somsen G W, Welten H T M E, Mulder F Ph, Swart C W, Kema I P, de Jong G J
Abstract excerpt
The use of capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection for the rapid determination of apolipoprotein E (apoE) genotypes was studied. High resolution and sensitive detection of the concerned DNA restriction fragments was achieved using CE buffers with hydroxypropylmethylcellulose (HPMC) as sieving polymer and ethidium bromide (EB) as fluorescent intercalating agent. In order to...
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