Article
Synthesis and application of charge-modified dye-labeled dideoxynucleoside-5'-triphosphates to 'direct-load' DNA sequencing.
Nucleic acids research - 1 Jul 2002
Finn Patrick J, Sun Lei, Nampalli Satyam, Xiao Haiguang, Nelson John R, Mamone J Anthony, Grossmann Greg, Flick Parke K, Fuller Carl W, Kumar Shiv
Abstract excerpt
A novel series of charge-modified, dye-labeled 2',3'-dideoxynucleoside-triphosphate terminators were synthesized and evaluated as reagents for DNA sequencing. These terminators possess an advantage over existing reagents in that no purification is required to remove unreacted nucleotide or associated breakdown products prior to electrophoretic separation of the sequencing fragments. This obviates the need for a...
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