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Article

Nuclei of nonviable ovine somatic cells develop into lambs after nuclear transplantation.

Biology of reproduction - 1 Jul 2002

Loi Pasqualino, Clinton Michael, Barboni Barbara, Fulka Josef, Cappai Pietro, Feil Robert, Moor Robert M, Ptak Grazyna

Abstract excerpt

Here we report on the successful reprogramming of nuclei from somatic cells rendered nonviable by heat treatment. Granulosa cells from adult sheep were heated to nonphysiological temperatures (55 degrees C or 75 degrees C) before their nuclei were injected into enucleated metaphase II oocytes. Reprogramming was demonstrated by the capacity of the reconstructed embryos to develop to the blastocyst stage in vitro...

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Identifiers

PMID: 12080008
DOI: 10.1095/biolreprod67.1.126

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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