Article
Recombinant forms of Gerbich blood group antigens: expression and purification.
Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine - 1 Apr 2002
Jaskiewicz E, Czerwinski M, Colin Y, Lisowska E
Abstract excerpt
Recombinant forms of normal glycophorin C (GPC), carrying the high frequency Gerbich blood group antigens, and its natural deletion mutants of Yus and Ge type (all combined with oligohistidyl tag) were expressed in CHO and COS 7 cells. The stable expression of all recombinant forms of GPC in CHO cells was obtained, but the level of expression was low and detectable only by flow cytometry. The high level of...
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