Article
A highly specific isolation of rat sinusoidal endothelial cells by the immunomagnetic bead method using SE-1 monoclonal antibody.
Journal of hepatology - 1 Jun 2002
Tokairin Takuo, Nishikawa Yuji, Doi Yuko, Watanabe Hitoshi, Yoshioka Toshiaki, Su Mu, Omori Yasufumi, Enomoto Katsuhiko
Abstract excerpt
BACKGROUND/AIMS: To develop a specific isolation method of hepatic sinusoidal endothelial cells (SEC), we applied the immunomagnetic method using a monoclonal antibody (SE-1) that recognizes a membranous antigen expressed only in rat SEC. METHODS: Cells were isolated by incubating mixed non-parenchymal cells, which were obtained by collagenase digestion of the liver, with SE-1-conjugated superparamagnetic...
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