Article
Advanced Method for Isolation of Mouse Hepatocytes, Liver Sinusoidal Endothelial Cells, and Kupffer Cells.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017
Liu Jia, Huang Xuan, Werner Melanie, Broering Ruth, Yang Dongliang, Lu Mengji
Abstract excerpt
Separation of pure cell populations from the liver is a prerequisite to study the role of hepatic parenchymal and non-parenchymal cells in liver physiology, pathophysiology, and immunology. Traditional methods for hepatic cell separation usually purify only single cell types from liver specimens. Here, we describe an efficient method that can simultaneously purify populations of hepatocytes (HCs), liver...
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