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Article

Role of Lys-32 residues in R67 dihydrofolate reductase probed by asymmetric mutations.

The Journal of biological chemistry - 5 Nov 2004

Hicks Stephanie N, Smiley R Derike, Stinnett Lori G, Minor Kenneth H, Howell Elizabeth E

Abstract excerpt

R67 dihydrofolate reductase (R67 DHFR) is a novel protein encoded by an R-plasmid that confers resistance to the antibiotic, trimethoprim. This homotetrameric enzyme possesses 222 symmetry, which imposes numerous constraints on the single active site pore, including a "one-site-fits-both" strategy for binding its ligands, dihydrofolate (DHF) and NADPH. Previous studies uncovered salt effects on binding and...

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Source

Identifiers

PMID: 15333636
DOI: 10.1074/jbc.M404484200

Genes

DHFR

Variants

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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