[Chinese little-fat-tail sheep prion protein gene belongs to PrPARH genotype].

Abstract excerpt

The genomic DNA was extracted from lymphocytes of peripheral blood of Chinese little-fat-tail sheep. The prion protein gene(PrPc) was amplified by polymerase chain reaction. The PCR products were cloned into pUC19 plasmid and was sequenced. The results indicated that 771 bp amplified fragment contains the entire sheep PrPc coding sequence. There were 7 base pairs different with the reported sheep PrPc sequence,...

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