Article
Characterization of yeast V-ATPase mutants lacking Vph1p or Stv1p and the effect on endocytosis.
The Journal of experimental biology - 1 May 2002
Perzov Natalie, Padler-Karavani Vered, Nelson Hannah, Nelson Nathan
Abstract excerpt
Subunit a of V-ATPase in the yeast Saccharomyces cerevisiae, in contrast to its other subunits, is encoded by two genes VPH1 and STV1. While disruption of any other gene encoding the V-ATPase subunits results in growth arrest at pH 7.5, null mutants of Vph1p or Stv1p can grow at this pH. We used a polyclonal antibody to yeast Stv1p and a commercially available monoclonal antibody to Vph1p for analysis of yeast...
Topics
- Endocytosis
- Fluorescent Dyes
- Genes, Fungal
- Hydrogen-Ion Concentration
- Mutation
- Phenotype
- Protein Subunits
- Saccharomyces cerevisiae
- Vacuolar Proton-Translocating ATPases
- Vacuoles