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Article

In vitro affinity screening of protein and peptide binders by megavalent bead surface display.

Protein engineering, design & selection : PEDS - 1 Oct 2013

Diamante Letizia, Gatti-Lafranconi Pietro, Schaerli Yolanda, Hollfelder Florian

Abstract excerpt

The advent of protein display systems has provided access to tailor-made protein binders by directed evolution. We introduce a new in vitro display system, bead surface display (BeSD), in which a gene is mounted on a bead via strong non-covalent (streptavidin/biotin) interactions and the corresponding protein is displayed via a covalent thioether bond on the DNA. In contrast to previous monovalent or low-copy...

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Identifiers

PMID: 23980186
DOI: 10.1093/protein/gzt039
PMCID: PMC3785251

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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