Development of a high-throughput label-free method for enrichment of antimetabolite-resistant yeast strains using pico-litre agarose beads.

Abstract excerpt

Antimetabolites, which are structural analogues of metabolites or carbon sources that exhibit toxicity by disrupting metabolism, can couple phenotypes of interest to growth and thereby facilitate the selection of relevant strains from large mutant pools generated by random mutagenesis. Toxicity and high costs of many antimetabolites provide incentives to minimise liquid volume during cultivation, while still...

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