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Article

Isolation of high-affinity ligand-binding proteins by periplasmic expression with cytometric screening (PECS).

Nature biotechnology - 1 Jun 2001

Chen G, Hayhurst A, Thomas J G, Harvey B R, Iverson B L, Georgiou G

Abstract excerpt

Periplasmic expression with cytometric screening (PECS) is a powerful and rapid "display-less" technology for isolating ligand-binding proteins from diverse libraries. Escherichia coli expressing a library of proteins secreted into the periplasmic space are incubated with a fluorescent conjugate of the target ligand. Under the proper conditions, ligands as large as about 10 kDa can equilibrate within the...

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PMID: 11385457
DOI: 10.1038/89281

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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