Article
DHPLC screening of cystic fibrosis gene mutations.
Human mutation - 1 Apr 2002
Ravnik-Glavac Metka, Atkinson Andrew, Glavac Damjan, Dean Michael
Abstract excerpt
Denaturing high performance liquid chromatography (DHPLC) using ion-pairing reverse phase chromatography (IPRPC) columns is a technique for the screening of gene mutations. In order to evaluate the potential utility of this assay method in a clinical laboratory setting, we subjected the PCR products of 73 CF patients known to bear CFTR mutations to this analytic technique. We used thermal denaturation profile...
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