Article
Validation of high-resolution DNA melting analysis for mutation scanning of the cystic fibrosis transmembrane conductance regulator (CFTR) gene.
The Journal of molecular diagnostics : JMD - 1 Sept 2008
Audrezet Marie-Pierre, Dabricot Aurélia, Le Marechal Cédric, Ferec Claude
Abstract excerpt
High-resolution melting analysis of polymerase chain reaction products for mutation scanning, which began in the early 2000s, is based on monitoring of the fluorescence released during the melting of double-stranded DNA labeled with specifically developed saturation dye, such as LC-Green. We report here the validation of this method to scan 98% of the coding sequence of the cystic fibrosis transmembrane...
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