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Article

Rapid detection of the deltaF508 mutation in single cells using DHPLC: implications for preimplantation genetic diagnosis.

Journal of assisted reproduction and genetics - 1 Apr 2003

Girardet Anne, Cathala Philippe, Claustres Mireille

Abstract excerpt

PURPOSE: Practice of preimplantation genetic diagnosis (PGD) requires efficient amplification and analysis techniques. We have tested Denaturing High Performance Liquid Chromatography (DHPLC) to screen the deltaF508 mutation in heterozygous single cells in order to assess its usefulness for PGD of cystic fibrosis. METHODS: One hundred and two single lymphocytes--including N/N and N/deltaF508--were studied. F508...

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Source

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Identifiers

PMID: 12762414
DOI: 10.1023/a:1022981103862
PMCID: PMC3455635

Genes

PGD
ADO

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Rapid detection of the deltaF508 mutation in single cells using DHPLC: implications for preimplantation genetic diagnosis.PMID 12762414Read ↗

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