Article
Large-scale analysis of gene expression, protein localization, and gene disruption in Saccharomyces cerevisiae.
Genes & development - 1 May 1994
Burns N, Grimwade B, Ross-Macdonald P B, Choi E Y, Finberg K, Roeder G S, Snyder M
Abstract excerpt
We have developed a large-scale screen to identify genes expressed at different times during the life cycle of Saccharomyces cerevisiae and to determine the subcellular locations of many of the encoded gene products. Diploid yeast strains containing random lacZ insertions throughout the genome have been constructed by transformation with a mutagenized genomic library. Twenty-eight hundred transformants containing...
Topics
- Base Sequence
- Cytoplasm
- Fluorescent Antibody Technique
- Fungal Proteins
- Gene Expression
- Genes, Fungal
- Meiosis
- Mitochondria
- Molecular Sequence Data
- Mutagenesis
- Open Reading Frames