Article
Secondary-site mutation restores the transport defect caused by the transmembrane domain mutation of the xenobiotic transporter MexB in Pseudomonas aeruginosa.
Biochemical and biophysical research communications - 29 Mar 2002
Yoneyama Hiroshi, Maseda Hideaki, Yamabayashi Ta Taka-aki, Izumi Shotaro, Nakae Taiji
Abstract excerpt
It has been suggested that the MexB subunit of the MexAB-OprM efflux transporter of Pseudomonas aeruginosa exports xenobiotics in an energy-dependent manner. To investigate the role of the transmembrane segments (TMS) of MexB in the transporter activity, we isolated 24 spontaneous mutants showing hypersusceptibility to antibiotics. Among them, three mutations were located at TMS-3, TMS-4, and TMS-10 having amino...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.