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Article

Functional cloning and characterization of a multidrug efflux pump, mexHI-opmD, from a Pseudomonas aeruginosa mutant.

Antimicrobial agents and chemotherapy - 1 Sept 2003

Sekiya Hiroshi, Mima Takehiko, Morita Yuji, Kuroda Teruo, Mizushima Tohru, Tsuchiya Tomofusa

Abstract excerpt

We isolated mutant YM644, which showed elevated resistance to norfloxacin, ethidium bromide, acriflavine, and rhodamine 6G, from Pseudomonas aeruginosa YM64, a strain that lacks four major multidrug efflux pumps. The genes responsible for the resistance were mexHI-opmD. Elevated ethidium extrusion was observed with cells of YM644 and YM64 harboring a plasmid carrying the genes. Disruption of the genes in the...

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Identifiers

PMID: 12937010
DOI: 10.1128/AAC.47.9.2990-2992.2003
PMCID: PMC182609

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Source receipt: pubmed, retrieved 7 Sept 2026.

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