Article
Substrate activation in acetylcholinesterase induced by low pH or mutation in the pi-cation subsite.
Biochimica et biophysica acta - 11 Feb 2002
Masson Patrick, Schopfer Lawrence M, Bartels Cynthia F, Froment Marie-Thérèse, Ribes Fabien, Nachon Florian, Lockridge Oksana
Abstract excerpt
Substrate inhibition is considered a defining property of acetylcholinesterase (AChE), whereas substrate activation is characteristic of butyrylcholinesterase (BuChE). To understand the mechanism of substrate inhibition, the pH dependence of acetylthiocholine hydrolysis by AChE was studied between pH 5 and 8. Wild-type human AChE and its mutants Y337G and Y337W, as well as wild-type Bungarus fasciatus AChE and...
Topics
- Acetylcholinesterase
- Acetylthiocholine
- Binding Sites
- Butyrylcholinesterase
- Humans
- Hydrogen-Ion Concentration
- Kinetics
- Models, Molecular
- Mutation
- Phenylalanine
- Protein Conformation
- Tyrosine