Article
Differential effect of pressure and temperature on the catalytic behaviour of wild-type human butyrylcholinesterase and its D70G mutant.
European journal of biochemistry - 1 Sept 1999
Weingand-Ziadé A, Renault F, Masson P
Abstract excerpt
The combined action of temperature (10-35 degrees C) and pressure (0. 001-2 kbar) on the catalytic activity of wild-type human butyrylcholinesterase (BuChE) and its D70G mutant was investigated at pH 7.0 using butyrylthiocholine as the substrate. The residue D70, located at the mouth of the active site gorge, is an essential component of the peripheral substrate binding site of BuChE. Results showed a break in...
Topics
- Binding Sites
- Butyrylcholinesterase
- Butyrylthiocholine
- Enzyme Stability
- Humans
- Hydrolysis
- Kinetics
- Mutation
- Pressure
- Protein Conformation
- Temperature