Article
A mutant of Sindbis virus with altered plaque morphology and a decreased ratio of 26 S:49 S RNA synthesis in mosquito cells.
Virology - 1 Jul 1991
Durbin R, Kane A, Stollar V
Abstract excerpt
When our stock of standard Sindbis virus (SVSTD) is assayed by plaque formation on Aedes albopictus mosquito cells, about 1-2% of the plaques appear much clearer and sharper than the majority of the plaques. One of these clear plaques was picked, grown into a viral stock (SVCP), and used to prepare viral cDNA. Making use of the infectious Sindbis virus plasmid, Toto 1101 (Rice et al., 1987), we mapped the causal...
Topics
- Aedes
- Animals
- Base Sequence
- Cells, Cultured
- Chick Embryo
- DNA, Viral
- Molecular Sequence Data
- Mutation
- Phenotype
- RNA, Ribosomal
- RNA, Viral
- Sindbis Virus