Article
[Establishment and identification of B cell lines with PNH phenotype].
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi - 1 Mar 2000
Li K, Shao Z, Kawaguchi T, Zhang Y, Zhao M, Wang L, Hao Y
Abstract excerpt
OBJECTIVE: To establish B cell lines from patients with paroxysmal nocturnal haemoglobinuria (PNH) and normal controls. METHODS: B lymphoid cells prepared from PNH patients and normal controls were infected with EB virus. Phenotype of the immortal cells was analyzed by flow cytometry. The immunomagnetic beads were used to select CD(55)(-) and CD(59)(-) B lymphoid cells, which were cloned by limiting dilution. The...
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