Article
Molecular basis and functional consequences of the dominant effects of the mutant band 3 on the structure of normal band 3 in Southeast Asian ovalocytosis.
Biochemistry - 12 Mar 2002
Kuma Hiroyuki, Abe Yoshito, Askin David, Bruce Lesley J, Hamasaki Tomohiro, Tanner Michael J A, Hamasaki Naotaka
Abstract excerpt
Southeast Asian ovalocytosis (SAO) human red cell membranes contain similar proportions of normal band 3 and a mutant band 3 with a nine amino acid deletion (band 3 SAO). We employed specific chemical modification and proteolytic cleavage to probe the structures of band 3 in normal and SAO membranes. When the membranes were modified specifically at lysine residues with N-hydroxysulfosuccinimide-SS-biotin, band 3...
Topics
- Amino Acid Sequence
- Anion Exchange Protein 1, Erythrocyte
- Biotin
- Chromatography, High Pressure Liquid
- Elliptocytosis, Hereditary
- Erythrocyte Membrane
- Erythrocytes, Abnormal
- Heterozygote
- Humans
- Hydrolysis
- Lysine
- Molecular Sequence Data