Article
[Comparison of the results of HLA typing using serologic and molecular genetics methods].
Bratislavske lekarske listy - 1 Jan 2000
Kulcsarova E, Kralovicova J, Parnicka Z, Ferencik S, Buc M
Abstract excerpt
A comparison of the HLA class I typing in 50 unrelated individuals by means of serological and molecular genetic (PCR-SSP) methods was carried out. DNA-typing is more fast and reliable method in comparison with serology. It is necessary to introduce molecular genetic methods for the detection of HLA class I alleles. On the other hand there are alleles, which are not expressed on cell surface. In our laboratory...
Topics
- Alleles
- Cytotoxicity Tests, Immunologic
- DNA Primers
- HLA Antigens
- Histocompatibility Testing
- Humans
- Polymerase Chain Reaction
- Slovakia