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Article

Selection of metalloenzymes by catalytic activity using phage display and catalytic elution.

Chembiochem : a European journal of chemical biology - 2 Apr 2001

Ponsard I, Galleni M, Soumillion P, Fastrez J

Abstract excerpt

The metallo-beta-lactamase betaLII from Bacillus cereus 569/H/9 was displayed on the filamentous phage fd. The phage-bound enzyme fd-betaLII was shown to be active on benzylpenicillin as substrate; it could be inactivated by complexation of the essential zinc(II) ion with EDTA and reactivated by addition of a zinc(II) salt. A selection process was designed to extract active phage-bound enzymes from libraries of...

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PMID: 11828452
DOI: 10.1002/1439-7633(20010401)2:4<253::AID-CBIC253>3.0.CO;2-6

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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