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Article

An altered zinc-binding site confers resistance to a covalent inactivator of New Delhi metallo-beta-lactamase-1 (NDM-1) discovered by high-throughput screening.

Bioorganic & medicinal chemistry - 1 Jun 2013

Thomas Pei W, Spicer Timothy, Cammarata Michael, Brodbelt Jennifer S, Hodder Peter, Fast Walter

Abstract excerpt

Due to the global threat of antibiotic resistance mediated by New Delhi metallo-beta-lactamase-1 (NDM-1) and the lack of structurally diverse inhibitors reported for this enzyme, we developed screening and counter-screening assays for manual and automated formats. The manual assay is a trans-well absorbance-based endpoint assay in 96-well plates and has a Z' factor of 0.8. The automated assay is an epi-absorbance...

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Identifiers

PMID: 23591260
DOI: 10.1016/j.bmc.2013.03.031
PMCID: PMC3651783

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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