Development of a rapid and sensitive high-performance liquid chromatographic method to determine CYP2D6 phenotype in human liver microsomes.

doi:10.1002/bmc.121

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Development of a rapid and sensitive high-performance liquid chromatographic method to determine CYP2D6 phenotype in human liver microsomes.

Biomedical chromatography : BMC - 1 Feb 2002

Rege Bhaskar, March Clark, Sarkar Mohamadi A

Abstract excerpt

Dextromethorphan is a probe substrate to determine CYP2D6 phenotype. The conversion of dextromethorphan to dextrorphan by CYP2D6 accounts for approximately 60% of total metabolism. Most analytical methods utilize complicated labor- and time-intensive sample processing methods with several liquid-liquid extraction (LLE) steps. Our goal was to develop a non-LLE based rapid and sensitive HPLC method, to measure...

Topics

Antitussive Agents
Chromatography, High Pressure Liquid
Cytochrome P-450 CYP2D6
Dextromethorphan
Humans
Kinetics
Microsomes, Liver
Phenotype
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Ultraviolet

Source

Open in PubMed

Identifiers

PMID: 11816009
DOI: 10.1002/bmc.121

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics