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Article

Kinetic analysis of C-terminally truncated RNA-dependent RNA polymerase of hepatitis C virus.

Biochemical and biophysical research communications - 1 Feb 2002

Kashiwagi Takahito, Hara Koyu, Kohara Michinori, Kohara Kyoko, Iwahashi Jun, Hamada Nobuyuki, Yoshino Haruhito, Toyoda Tetsuya

Abstract excerpt

The biochemical properties of hepatitis C virus (HCV) RNA-dependent RNA polymerase (RdRp) truncated with C-terminal 21 amino acids and expressed in insect cells were analyzed. The enzyme carried copy-back and de novo RNA synthesis activity but not terminal nucleotidyl transferase activity. k(pol) and K(m) for de novo RNA synthesis were calculated as 10.0 pmol/microg/h and 2.5 microM under 0.5 mM GTP and 2.0...

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Identifiers

PMID: 11811988
DOI: 10.1006/bbrc.2001.6333

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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