Article
Ordered catenation of sequence-tagged sites and multiplexed SNP genotyping by sequencing.
Nucleic acids research - 1 Feb 2002
Higasa Koichiro, Hayashi Kenshi
Abstract excerpt
We describe a method for the efficient genotyping of SNPs, involving sequencing of ordered and catenated sequence-tagged sites (OCS). In OCS, short genomic segments, each containing an SNP, are amplified by PCR using primers that carry specially designed extra nucleotides at their 5'-ends. Amplification products are then combined and converted to a concatamer in a defined order by a second round of thermal...
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